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Zymoclean Gel RNA Recovery Kit
Highlights
Quick and Reliable: 30 minute recovery of purified RNA fragments from agarose gels.
Concentrated: Up to 10 µg sample in ≥ 6 µl elution.
High Recovery: ≥ 80% recovery for RNA > 500 nt.
서한형 대리

Zymo Research 제품 담당자

경신과학(주)

영업부

H.P) 010-8832-6303

HanHyung Seo

Zymo Research Brand Manager

Kyongshin scientific Co., Ltd.

Sales Department

H.P) 82)10-8832-6303

제품소개

Highlights

  • Quick and Reliable: 30 minute recovery of purified RNA fragments from agarose gels.
  • Concentrated: Up to 10 µg sample in ≥ 6 µl elution.
  • High Recovery: ≥ 80% recovery for RNA > 500 nt.


 

Product Description


The Zymoclean Gel RNA Recovery Kit provides a quick and efficient purification method for recovery of RNA fragments from agarose gels. The procedure combines a unique, single-step agarose dissolving/RNA binding buffer with Zymo-Spin column technology to yield high quality, purified RNA in just minutes. The purified RNA is eluted into small volumes of DNase/RNase-free water for highly concentrated samples suitable for subsequent RNA-based manipulations. Compatible with MOPS, TAE, and TBE buffered agarose gels (formaldehyde up to 2.0%).

Technical Specifications

EquipmentMicrocentrifuge, 37°C-65°C heat source
PurityRNA is ready for all subsequent analysis and molecular manipulation
Sample SourceSingle- or double-stranded RNA fragments (≥200 nt) resolved in TAE TBE, and MOPS buffered agarose gels. Compatible with formaldehyde to 2.0% (final conc.)
Size RangeRNA fragments ≥500 nt (≥80% recovery)
Yield10 µg RNA (binding capacity), ≥6 µl (elution volume)

Resources


Q1: Are the columns and wash buffer interchangeable with the RCC-5 Kit?

Yes, the Zymo-Spin IC Columns and RNA Wash Buffer are interchangeable.

Q2: Can the Zymoclean be used for samples in solution (without running on a gel)?

No, it’s not optimized for samples in solution. High efficiency recovery is not guaranteed and the success of the purification can be variable.

Q3: What is the maximum weight of gel slice I can use?

Up to 400 mg of agarose.

Q4: I ran out of RNA Wash Buffer. Can I use something else?

Yes. Use 80% ethanol as a substitute. RNA Wash Buffer is also sold separately.

Q5: Will this kit remove fluorescent dyes, radiolabeled dNTP’s and/or Biotin?

Yes, the kit efficiently removes unincorporated fluorescent dyes, radiolabeled dNTP’s and Biotin.

주문정보

CAT.No 품명 규격 비고
R1011 Zymoclean Gel RNA Recovery Kit 50 preps.