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ZR small-RNA PAGE Recovery Kit
Highlights
Efficient: Recovery of ss- or ds- RNA (and DNA) fragments (17-200 nt) from up to 20% (w/v) polyacrylamide gels.
Concentrated: Up to 10 µg sample in ≥ 6 µl elution.
Compatible with up to 25% (w/v) polyacrylamide.
서한형 대리

Zymo Research 제품 담당자

경신과학(주)

영업부

H.P) 010-8832-6303

HanHyung Seo

Zymo Research Brand Manager

Kyongshin scientific Co., Ltd.

Sales Department

H.P) 82)10-8832-6303

제품소개

Highlights

  • Efficient: Recovery of ss- or ds- RNA (and DNA) fragments (17-200 nt) from up to 20% (w/v) polyacrylamide gels.
  • Concentrated: Up to 10 µg sample in ≥ 6 µl elution.
  • Compatible with up to 25% (w/v) polyacrylamide.


 

Product Description

The ZR small-RNA PAGE Recovery Kit provides an easy and efficient method for the extraction of high quality small RNAs from polyacrylamide gels (native and/or denatured). The ZR small-RNA™ PAGE Recovery Kit is a refinement of the "crush and soak" method that incorporates a unique buffer system together with Zymo-Spin column technologies for improved recovery and added convenience. The recovered RNA can be concentrated into volumes as small as 6 µl, making it ideal for many downstream enzymatic reactions and manipulations.

Technical Specifications

EquipmentMicrocentrifuge, 37°C-65°C heat source, dry ice or -80°C freezer
PurityRNA is ready for all subsequent analysis and molecular manipulation
Sample SourceRNA or DNA fragments (17-200 nt) resolved in PAGE gels (up to 25%), also compatible with TBE-, denatured, urea PAGE gels. Compatible with ethidium bromide or ssRNA-specific dyes (e.g. GelStar).
Size RangeRNAs 17-200 nt (fragments 17-28 nt is ≥50 % recovery)
Yield10 µg RNA (binding capacity), ≥6 µl (elution volume)

Resources

Q1: What is the recovery rate?

The recovery rate for fragments 17 to 28 nucleotides is ≥50 %.

Q2: Can the kit be used to recover DNA fragments run on a PAGE gel?

Yes, the kit is compatible with both single- or double-stranded RNA (and DNA) fragments.

Q3: Can the kit be used to purify DNA/RNA fragments > 200 nucleotides run on a PAGE gel?

Yes. Most customer’s do not use PAGE gels for to visualize fragments > 200 nucleotides, but it will be compatible with the kit.

Q4: Is the kit compatible with TBE-Urea PAGE?

Yes, this kit is compatible with TBE-Urea PAGE gels.

Q5: What is the maximum amount of gel that can be processed per prep?

There is no designated upper limit, however it is always more optimal to start with keeping the amount of excised gel as low as possible prior to proceeding with the purification procedure, and scaling up when optimizing gel input amount.

Q6: Will this kit remove fluorescent dyes, radiolabeled dNTP’s and/or Biotin?

Yes, the kit efficiently removes unincorporated fluorescent dyes, radiolabeled dNTP’s and Biotin.

주문정보

CAT.No 품명 규격 비고
R1070 ZR small-RNA PAGE Recovery Kit 20 preps.