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Quick-cfDNA/cfRNA Serum & Plasma Kit

Highlights
Quick & Easy: Simple spin-column based isolation from up to 3 mL of plasma, serum, or other biological liquids. No phenol/chloroform or precipitation.
Highest Yields: Efficient isolation of cell-free DNA and RNA. Recover up to 515 times more microRNA.
Ultra-Pure: Ready for Next-generation sequencing, RT-qPCR, nCounter, etc.

서한형 대리

Zymo Research 제품 담당자

경신과학(주)

영업부

H.P) 010-8832-6303

HanHyung Seo

Zymo Research/Bertin Bioreagent Brand Manager

Kyongshin scientific Co., Ltd.

Sales Department

H.P) 82)10-8832-6303

제품소개

Highlights

Quick & Easy: Simple spin-column based isolation from up to 3 mL of plasma, serum, or other biological liquids. No phenol/chloroform or precipitation.
Highest Yields: Efficient isolation of cell-free DNA and RNA. Recover up to 515 times more microRNA.
Ultra-Pure: Ready for Next-generation sequencing, RT-qPCR, nCounter, etc.

Product Description

The Quick-cfDNA/cfRNA Serum & Plasma Kit enables simple and efficient preparation of all circulating cell-free DNA and RNA (including protein-bound, exosomal, microRNA, and other small RNA) from serum, plasma, and other biological fluids, without using toxic phenol-chloroform or cumbersome protein precipitation and removal steps. The kit efficiently separates cell-free DNA and RNA, with option to co-elute. Cell-free nucleic acids scale linearly relative to the sample input volume up to 3 ml. Zymo-Spin™ Technology enables isolation of ultra-pure cell-free nucleic acids suitable for all subsequent analyses, such as RT-qPCR and NGS.

Technical Specifications

Elution Volume	cfDNA and cfRNA can be eluted into 15 µl (to maximize total yield) or as little as 6 µl (for highly concentrated eluate) of DNase/RNase-Free Water.
Processing Time	Typically requires 30 minutes (cfDNA/cfRNA co-elution protocol) to 45 minutes (cfDNA/cfRNA separation protocol) hands-on time to process 10 samples. Sample digestion requires 2 hours.
Purity	High quality cell-free DNA (cfDNA) and cell-free RNA (cfRNA) are ready for all sensitive downstream applications such as RT-qPCR and Next-Generation Sequencing.
Required Equipment	Microcentrifuge, vortex, water bath or heating block, vacuum, and vacuum manifold.
Sample Types	Up to 3 ml of plasma, serum, cerebrospinal fluids, amniotic fluids, urine, or other biological fluids.
Size	DNA ≥ 50 bp and RNA ≥ 17 nt.
Yield	Yields are sample dependent and may vary. Typical recovery ranges from 1 – 100 ng/ml of human plasma or serum for both cfDNA and cfRNA. Kit is optimized for recovery of total cfDNA and cfRNA, including small RNA and microRNA.

주문정보

CAT.No	품명	규격	비고
R1072	Quick-cfDNA/RNA Serum & Plasma Kit	50 preps