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CpG Methylase (M. Sssl)
Highlights
For complete, in vitro methylation of DNA for methylation analysis.
Methylation of chromatin DNA for DNA accessibility studies.
Inhibition of endonucleases with overlapping CpG sequence recognition and [3H]-labeling of DNA.
서한형 대리

Zymo Research 제품 담당자

경신과학(주)

영업부

H.P) 010-8832-6303

HanHyung Seo

Zymo Research Brand Manager

Kyongshin scientific Co., Ltd.

Sales Department

H.P) 82)10-8832-6303

제품소개

Highlights

  • For complete, in vitro methylation of DNA for methylation analysis.
  • Methylation of chromatin DNA for DNA accessibility studies.
  • Inhibition of endonucleases with overlapping CpG sequence recognition and [3H]-labeling of DNA.

Product Description

CpG Methylase completely methylates all cytosines in double-stranded, non-methylated, and hemimethylated DNA in a 5'...CpG...3' context. The recombinant methylase is isolated from an E. coli strain that expresses the methyltransferase gene from Spiroplasma sp. strain MQ1. Reaction conditions have been optimized to maximize the processivity of the enzyme to ensure rapid, complete, and reproducible methylation of DNA for accurate DNA methylation analysis. This product is supplied with 10X Reaction Buffer and S-adenosylmethionine cofactor.

Technical Specifications

Additional InformationEnzyme Commission Number (EC 2.1.1.37)
Assay ConditionCpG Methylase in 1X CpG Reaction Buffer w/ 600 µM SAM. Incubate reaction mixtures at 30C (Performing the Methylase reaction at 30°C rather than 37°C promotes optimal reaction kinetics).
Heat Inactivation65°C for 20 minutes
SourceRecombinant methylase is isolated from E. coli expressing the methyltransferase gene from Spiroplasma sp. strain MQ1.
StorageStore at -20°C for up to 12 months. Avoid repeated freeze/thaw cycles of reagents. Prolonged storage is at ≤ -70°C.
Unit DefinitionOne unit (U) is the amount of enzyme required to protect 1µg of λ DNA from cleavage by BstUI restriction endonuclease in a total reaction volume of 20 µl for 1 hour at 37°C.

주문정보

CAT.No 품명 규격 비고
E2010 CpG Methylase (M. Sssl) 200 U
E2011 CpG Methylase (M. Sssl) 400 U