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dsDNA Shearase Plus (Includes Matching DCC-5 Kit)
Highlights
The simplest method for generating random-ended dsDNA fragments. Fragment size can be controlled by adjusting the enzyme concentration.
Included DNA Clean & Concentrator-5 (DCC-5) kit allows for easy clean-up of fragmented DNA.
Fragmented DNA is ideal for downstream applications such as library construction, Next-Gen sequencing, MeDIP, MeDIP-Seq, etc.
서한형 대리
Zymo Research 제품 담당자
경신과학(주)
영업부
H.P) 010-8832-6303
HanHyung Seo
Zymo Research Brand Manager
Kyongshin scientific Co., Ltd.
Sales Department
H.P) 82)10-8832-6303
제품소개
Highlights
- The simplest method for generating random-ended dsDNA fragments. Fragment size can be controlled by adjusting the enzyme concentration.
- Included DNA Clean & Concentrator-5 (DCC-5) kit allows for easy clean-up of fragmented DNA.
- Fragmented DNA is ideal for downstream applications such as library construction, Next-Gen sequencing, MeDIP, MeDIP-Seq, etc.
dsDNA Shearase Plus is an endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5'-phosphates and 3’-hydroxyl termini. It has a particularly strong preference for dsDNA and generates random-ended DNA fragments of the desired size in a single step. This enzyme is compatible with low volume inputs thus minimizing sample loss.
| Concentration | 1 U/µl |
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| Enzyme Inactivation | Heat in activate enzyme at 65C for 5 min. |
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| Storage | Store at -20C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage is at ≤ -70C. |
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| Unit Definition | One unit (1 U) is defined at the amount of enzyme required to convert 250 ng human DNA into DNA fragments in the range of 100-500 bp in 20 minutes at 42C in total reaction volume in 10 µl. |
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주문정보
| CAT.No |
품명 |
규격 |
비고 |
| E2019-50 |
dsDNA Shearase Plus (Includes Matching DCC-5 Kit) |
50 U |
|
| E2019-200 |
dsDNA Shearase Plus (Includes Matching DCC-5 Kit) |
200 U |
|
