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5-Fluoroorotic Acid (5-FOA)
Highlights
Yeast Genetic Counter-Selection Agent: Commonly used for curing yeast strains of plasmids, plasmid shuffling, allelic replacement, and two-hybrid screens.
Convenient: Available as a pure powder or ready-to-use solution in DMSO.
Ultra-Pure: Determined > 98% by thin-layer chromatography (TLC), melting point, and lot comparison.
서한형 대리
Zymo Research 제품 담당자
경신과학(주)
영업부
H.P) 010-8832-6303
HanHyung Seo
Zymo Research Brand Manager
Kyongshin scientific Co., Ltd.
Sales Department
H.P) 82)10-8832-6303
제품소개
Highlights
- Yeast Genetic Counter-Selection Agent: Commonly used for curing yeast strains of plasmids, plasmid shuffling, allelic replacement, and two-hybrid screens.
- Convenient: Available as a pure powder or ready-to-use solution in DMSO.
- Ultra-Pure: Determined > 98% by thin-layer chromatography (TLC), melting point, and lot comparison.
Using 5-Fluoroorotic Acid (5-FOA) for the counter-selection of yeast is a common genetic screening method. Curing yeast strains of plasmids, plasmid shuffling, allelic replacement, and two-hybrid screens are methods that can employ the use of 5-FOA. Otherwise nontoxic to yeast, 5-FOA is converted to the toxic form (i.e., 5-flurouracil) in strains expressing the functional URA3 gene coding for orotine-5-monophosphate decarboxylase that is involved in the synthesis of uracil. Yeast strains that are phenotypically Ura+ become Ura- and 5-FOA(R) (resistant) after selection. The question of 5-FOA solubility is often raised by customers using ultra-pure (< 98%) 5-FOA powder because of its insolubility in water. Thus, we provide a 100X concentrated (100 mg/ml) 5-FOA solution in DMSO and synthetic 5-FOA monohydrate powder. Both have been tested and validated on the basis of counter selection activity.
| Format | Synthetic 5-FOA monohydrate powder or 100X liquid (100 mg/ml in DMSO) |
|---|
| Molecular Weight | 174 g/mol |
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| Product Storage | Store at -20°C |
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| Purity | ≥ 98% (estimated) by TLC, melting point, and lot comparison |
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| Solubility | 50 mg in 1 ml (1:1 1:1 NH4 OH:H2O) with gentle heating; >100 mg/ml in DMSO |
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| Structure | C5H3FN2O4• H2O |
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주문정보
| CAT.No |
품명 |
규격 |
비고 |
| F9001-1 |
5-Fluoroorotic Acid (powder) |
1 g |
|
| F9001-5 |
5-Fluoroorotic Acid (powder) |
5 g |
|
| F9003 |
100X 5-Fluoroorotic Acid (Liquid) |
10 ml |
|
