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Quick-RNA Viral 96 Kit
Highlights
Quick, high-throughput (96-well spin plate) purification of viral RNA from plasma, serum, CSF, cell culture media, cellular suspensions, urine, blood, saliva, swab, fecal, etc.
Omits the use of organic denaturants and proteases.
High-quality viral RNA is ready for RT-PCR, sequencing, etc.
서한형 대리

Zymo Research 제품 담당자

경신과학(주)

영업부

H.P) 010-8832-6303

HanHyung Seo

Zymo Research Brand Manager

Kyongshin scientific Co., Ltd.

Sales Department

H.P) 82)10-8832-6303

제품소개

Highlights

  • Quick, high-throughput (96-well spin plate) purification of viral RNA from plasma, serum, CSF, cell culture media, cellular suspensions, urine, blood, saliva, swab, fecal, etc.
  • Omits the use of organic denaturants and proteases.
  • High-quality viral RNA is ready for RT-PCR, sequencing, etc.
 

Description

The Quick-RNA Viral 96 Kit provides for rapid, large scale isolation of viral RNA from plasma, serum, cell culture media, cellular suspensions, urine, blood, saliva and any other biological samples stored in DNA/RNA Shield. DNA/RNA Shield ensures nucleic acid stability during sample storage/transport at ambient temperatures. The reagent effectively lyses cells and inactivates nucleases and infectious agents (virus). The kit features a specialized buffer system that facilitates complete viral particle lysis for efficient RNA isolation from samples containing enteroviruses, rhinoviruses, coronaviruses, HIV, HCV, influenza A virus, flaviviruses, measles virus, parainfluenza virus, parvovirus (a ssDNA virus), etc. Viral RNA is bound to the matrix in each well of the spin-plate, washed and eluted. The isolated high-quality viral RNA is ready for all downstream applications such as Next-Gen sequencing, hybridization-based and RT/PCR detection.

Performance

Technical Specifications
EquipmentCentrifuge with microplate carriers with height tolerance of 60 mm (2.36 inches)
PurityRNA is ready for Next-Gen sequencing, RT-qPCR, microarray, hybridization, etc.
Registration StatusCE-IVD certified (R1040-E, R1041-E)
Sample SourcePlasma, serum, saliva, urine, blood, cell culture media, cellular suspensions, biopsies, swab and fecal samples
Size Range50 nt to ~200 kb
YieldSpin plate, 10 µg RNA (binding capacity), ≥10 µl (elution volume)

Resources

Protocol: R1040/R1041 | R1040E/R1041E
Datasheet: 
SDS (MSDS): EU | US

FAQ

The purpose of beta-mercaptoethanol is to help with deproteination. It is not necessary if you are working with simple samples such as swabs. It is recommended if you are working with protein rich samples such as plasma, serum, blood, saliva, sputum, etc.

Yes, these samples are compatible.

Yes, this kit will co-purify some DNA.

Most downstream application methods for viral detection do not require DNase treatment during purification. If necessary, DNase treatment can be perform post-purification and additional components can be purchased separately (i.e., DNase I, DNA Digestion Buffer, RNA Prep Buffer and RNA Wash Buffer).

Yes, the change from clear to yellow is a result of oxidation and will not affect the performance of the buffer.

The Viral RNA Buffer is guaranteed for one year from the date of purchase even with the addition of beta-mercaptoethanol. Just be sure to close the bottle cap tightly to prevent evaporation.

In most cases, it could be because the samples contain high amounts of protein or cellular debris. To help prevent this in future preps, it’s best to add beta-mercaptoethanol to the Viral RNA buffer, and/or implement a Proteinase K digestion step.

For optimal results and detection of viral target, collect sample in DNA/RNA Shield and perform Proteinase K treatment. In addition, add beta-mercaptoethanol to the Viral RNA Buffer prior to purification, as well as perform all steps at room temperature and centrifugation speeds at 10,000-16,000 x g to ensure no buffer retention.

The “-E" markings at the end of the catalog numbers of “-Dx” products indicate that they are CE-IVD verified for in vitro diagnostic use.

This can happen on occasion due to transport or storage at lower temperatures. The reagent functionality is not affected; however, the precipitate can be resolved by heating the reagent to >37 °C.

Reviews

"The Quick-RNA Viral RNA kit performed much better than the RNeasy 96 kit for extracting RNA from dilute samples of virus supernatant. The kit is designed for small-volume elution to automatically concentrate the sample, and it’s designed for viral supernatants rather than cell lysates, so we didn’t need to deal with optimizing added carrier RNA. So in all, it was simpler, easier, and gave us a 10-fold improvement in our limit of detection."

S.B.

Boehringer Ingelheim, Canada

주문정보

CAT.No 품명 규격 비고
R1040 Quick-RNA Viral 96 Kit 2 x 96 preps
R1041 Quick-RNA Viral 96 Kit 4 x 96 preps
R1040-E Quick-RNA Viral 96 Kit - DX 2 x 96 Preps
R1041-E Quick-RNA Viral 96 Kit - DX 4 x 96 Preps