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RNA Clean & Concentrator-100
Highlights
NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc.
Ultra-Pure: Eliminate contaminants and inhibitors in 5 minutes.
Maximum Recovery: Recover > 90% and elute in as little as 100 µl.
서한형 대리

Zymo Research 제품 담당자

경신과학(주)

영업부

H.P) 010-8832-6303

HanHyung Seo

Zymo Research Brand Manager

Kyongshin scientific Co., Ltd.

Sales Department

H.P) 82)10-8832-6303

제품소개

Highlights

  • NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc.
  • Ultra-Pure: Eliminate contaminants and inhibitors in 5 minutes.
  • Maximum Recovery: Recover > 90% and elute in as little as 100 µl.

Description

The RNA Clean & Concentrator-100 kit are RNA clean up kits that provide a simple and reliable method for the rapid preparation of high-quality, RT-PCR-ready RNA. This simple procedure is based on the use of a unique single-buffer system and Zymo-Spin column technology that allows for selective recovery of total RNA (> 17 nt), large RNAs (> 200 nt), and/or small RNAs (17-200 nt). The procedure is easy: Add binding buffer and ethanol to your sample, then bind, wash and elute ultra pure RNA. The RNA can be eluted from the Zymo-Spin V-E Column in as little as ≥ 100 µl of RNase-free water. The highly-concentrated, purified RNA is suitable for all subsequent analyses and molecular manipulations.

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Performance

Technical Specifications
EquipmentMicrocentrifuge and Vacuum Manifold
FormatSpin-Column
PurityRNA is ready for Next-Gen sequencing, RT-PCR, microarray, hybridization, etc. A260/A280, A260/A230: > 1.8.
Sample SourceDNase I treated RNA, in vitro transcription products, the aqueous phase following TRIzol/chloroform or similar extraction
Size RangeTotal RNA ≥ 17 nt
Yield1 mg RNA (binding capacity), ≥ 100 µl (elution volume)

Resources

Protocol: 
Datasheet: 
SDS (MSDS): 

FAQ

Yes. The kit efficiently recovers all types of nucleic acids.

Carrier RNA can be added with no detrimental effects. The RCC is highly efficient without the need for carrier RNA, and no significant improvement in recovery has been observed with carrier RNA.

Yes, the kit efficiently removes unincorporated fluorescent dyes, radiolabeled dNTP’s and Biotin.

Product Tolerance Reference: – ≤5% Triton X-100 – ≤5% Tween-20 – ≤5% Sarkosyl – ≤0.1% SDS – ≤90% formamide – Sucrose samples should be diluted/titrated down 10- to 100- fold.

Yes, follow respective protocol for on-column DNase treatment. If the DNase does not have a protocol, proceed with in-tube DNase treatment post clean-up, then re-purify.

If the downstream application requires DNA-free RNA, we would recommend performing in-column or in-tube DNase I treatment.

Both treatments will remove DNA efficiently. For pre-extracted RNA, perform in-tube DNase treatment followed by RNA clean-up.

Reviews

"Great for purifying small amounts of RNA. I use a bioanalyzer after and I can see the difference from before, cleaner bands and the concentration increases with a smaller amount of elutant."

Catherine A.

USAMRIID

"Kit was easy and fast, and cleaned up my RNA samples very well. It did exactly what I needed it to do."

Caroline S.

Cornell University

"The product is very easy to use, very quick protocol for an excellent RNA clean up. It also purifies small RNA which is not the case of all RNA clean up columns."

Agnes N.

Biofidal

주문정보

CAT.No 품명 규격 비고
R1019 RNA Clean & Concentrator-100 25 preps